CHRAC1 Protein Lysate (Rat) with C-HA Tag | Chromatin Remodeling Research

The gene CHRAC1 encodes a small histone-fold protein known as Chromatin Accessibility Complex Subunit 1 (CHRAC1). CHRAC1 plays a key role in chromatin structure regulation, DNA accessibility and nucleosome remodelling. For researchers working on chromatin biology, epigenetics, replication or transcription, having a high-quality lysate reagent (such as CHRAC1 Protein Lysate (Rat) with C-HA Tag) enables focused investigation of CHRAC1 protein behaviour under physiologic or experimental conditions.

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Protein identity & family

-CHRAC1 is part of the chromatin accessibility (CHRAC) complex (Wkipedia), which includes the ISWI/SNF2H ATP-dependent remodeler, BAZ1A/ACF1 and POLE3.

-The CHRAC1 protein contains a histone-fold domain, enabling binding to naked DNA and incorporation into chromatin architecture.

-In the rat gene context, orthologs exist and the gene has been annotated in RGD (Rat Genome Database) under RGD ID 1309321.

Image from GeneCards

Cellular localisation & role

-CHRAC1 is primarily nuclear, as part of the chromatin-remodelling assembly. Uniprot

-In DNA replication through heterochromatin and nucleosome repositioning contexts, CHRAC1 contributes to efficient progression of replication forks and transcription by facilitating nucleosome sliding.

Pathophysiological relevance

-Altered expression of CHRAC1 has been linked with cancer phenotypes (for example enhancing tumour growth via YAP transcription activity) in recent studies. ResearchGate

-Given its core role in chromatin and nucleosome positioning, CHRAC1 may influence epigenetic regulation, genomic stability and cellular lineage decisions.

Product reference: CHRAC1 Protein Lysate (Rat) with C-HA Tag (Gentaur)

Researchers interested in CHRAC1 may use this lysate for downstream applications such as western blot, immunoprecipitation, pull-down assays, functional assays of chromatin complexes, or over-expression and interaction studies in rodent systems.

Because the lysate uses rat origin and includes a C-terminal HA tag, it allows for convenient detection via anti-HA antibodies and compatibility with rodent cell systems.

How this reagent supports research :

-Protein–protein interaction mapping : Use the HA-tag to immunoprecipitate CHRAC1 and identify binding partners (e.g., POLE3, ISWI, BAZ1A) in rat cells or lysates.

-Chromatin remodelling assays : Apply the rat lysate in nucleosome sliding or accessibility assays to probe how CHRAC1-containing complex influences DNA accessibility.

-Knock-in/overexpression controls : Compare native vs tagged CHRAC1 expression in functional assays of replication, transcription or chromatin compaction.

-Comparative species work : The rat version may be useful in rodent models for translational studies bridging human biology and animal models.

Sample system and species relevance

-Since this product is rat (Rattus norvegicus) derived, confirm that your experimental system (cell line, tissue, model) is compatible with rat CHRAC1 or cross-reactivity of human/rodent orthologs is acceptable. The rat gene has been annotated.

-Consider whether the endogenous rat expression levels, post-translational modifications or interacting partners align with your research questions.

Tag interference and detection

-The C-HA tag facilitates detection but ensure that the tag does not interfere with CHRAC1 function in your assay (for example binding to DNA or interacting partners). Controls with untagged or native CHRAC1 may be helpful.

Assay optimization

-Validate the antibody specificity for HA and CHRAC1 in your system, especially to avoid cross-reactivity.

-In chromatin or nucleosome-remodelling assays, ensure that the presence of the tag and lysate matrix do not alter baseline activity.

-For immunoprecipitation or pull-down, consider using proper controls (e.g., HA-tag only, empty lysate) to identify non-specific binding.

Data interpretation

When using lysate reagents, be aware of endogenous protein background, potential degradation fragments, and variability in post-translational states. Since CHRAC1’s function is tightly linked to protein complex architecture and DNA binding, assay conditions (salt, buffer, DNA substrate) must be calibrated.

CHRAC1 is a critical component of the CHRAC chromatin-remodelling complex. For researchers working on chromatin structure, DNA replication, transcriptional regulation or epigenetics, having access to the CHRAC1 Protein Lysate (Rat) with C-HA Tag enables numerous functional studies, from interaction mapping to chromatin accessibility assays. Careful assay design, species-match consideration and appropriate controls will maximize the value of this reagent in your experimental workflow.

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