CKM Native Protein : Creatine Kinase Muscle Native Protein - Native Creatine Research Guide

The CKM native protein : Creatine Kinase Muscle Native Protein is a critical enzyme in the study of native creatine metabolism, widely used in biomedical and diagnostic research. As a naturally derived enzyme from human cardiac tissues, it plays a central role in cellular energy regulation.

The growing interest in native creatine research highlights the importance of using biologically active proteins like CKM to ensure accurate experimental outcomes

The CKM native protein : Creatine Kinase Muscle Native Protein is a muscle-specific isoenzyme responsible for catalyzing the reversible transfer of phosphate between ATP and creatine.

Here is the core biochemical reaction:

Creatine+ATP↔Phosphocreatine+ADP

This reaction is essential for rapid ATP regeneration in high-energy tissues such as skeletal and cardiac muscle.

1. Product Name: CKM native protein : Creatine Kinase Muscle Native Protein
2. Source: Human cardiac tissues
3. Molecular Weight: 82 kDa
4. Isoelectric Point (pI): 5.0 – 7.5
5. Isoenzyme Type: CK-MM (muscle-specific)

The enzyme belongs to the native creatine kinase family, which includes:

1. CK-MM (muscle)
2. CK-MB (cardiac)
3. CK-BB (brain)

Using native creatine proteins like CKM ensures:

1. Accurate enzyme kinetics
2. Physiological relevance in experiments
3. Reliable biomarker calibration
4. Better reproducibility in assays

Unlike recombinant or denatured proteins, native creatine enzymes preserve their natural structure and activity.

The CKM native protein : Creatine Kinase Muscle Native Protein is widely used in:

Diagnostic Applications

1. Calibration of clinical chemistry analyzers
2. Detection of muscle and cardiac damage
3. Biomarker validation studies

Research Applications

1. Neuromuscular disease research
2. Mitochondrial dysfunction analysis
3. Drug development targeting energy metabolism

Disease Studies

CKM levels are altered in many conditions, including:

1. Muscular dystrophy
2. Amyotrophic lateral sclerosis (ALS)
3. Polymyositis
4. Rhabdomyolysis
5. Myocardial injury
6. Thyroid disorders

CKM Native Protein Details

1. Purity: >50%
2. Buffer Composition:50 mM Tris
3. 2 mM DTT
4. pH 7.5
5. 50% Glycerol

This optimized formulation ensures enzyme stability and activity for advanced research applications.

To maintain the integrity of the CKM native protein : Creatine Kinase Muscle Native Protein:

1. Stable at 14°C for up to 1 week
2. Recommended storage: 2°C to 8°C
3. Avoid repeated freeze-thaw cycles

Proper storage is essential for preserving native creatine enzyme activity.

The CKM native protein : Creatine Kinase Muscle Native Protein is an essential tool for researchers studying native creatine metabolism, muscle physiology, and disease biomarkers.

Its natural origin, biochemical accuracy, and wide application range make it a valuable asset in both clinical and research laboratories.