Adenovirus Purification from any input – cell fraction or media fraction Rapid purification within 2 to 4.5 hours No specialized equipment needed (ultracentrifuge not required) Purify adenovirus cell culture supernatant from 1 mL to 33.5 mL input per prep Purify adenovirus cell pellet from 1 mL of input per prep Up to 25X sample concentration Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Adenoviral vectors are useful tools for both in vitro and in vivo gene transfer, and oncolytic viruses based on adenovirus are highly promising for cancer treatment. Norgen’s Adenovirus Purification Kit provides a fast and simple procedure for concentrating and purifying adenoviral vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. Adenoviral vectors purified in this manner are highly active for use in transduction experiments. Norgen’s Adenovirus Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant or a total of 500 mL of supernatant input) . Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit.