BRFF-BMZERO™ is a complete serum-free medium comprised of essential amino acids vitamins inorganic salts trace elements and growth factors designed for culturing human breast cell lines. BMZERO™ was developed and has been extensively used for establishing new epithelial cell lines from explants of human breast tissue and to grow immortalized normal breast cell lines. Optimal attached monolayer cell growth can be achieved when used in conjunction with FNC Coating Mix®.