No wash calcium assay, 10 plates. Total assay solution for plate-based, high-throughput measurements of intracellular calcium flux. Excitation: 490 nm | Emission: 515 nm ION Biosciences Brilliant Calcium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes intracellular Ca2+ mediated through a wide-variety of plasma membrane and intracellular calcium channels and transporters. The ION Biosciences Brilliant Calcium Assay is also useful for investigating numerous effectors of ion channels and transporters including G protein-coupled receptors, lipid kinases and protein kinases. In multi-well, plate-based formats, the Brilliant Calcium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Ca2+. For the last 25 years, fluorescence-based measures of Ca2+ flux have brought about the discovery of small-molecule modulators of a host of Ion channels, transporters, GPCRS and other targets of interest for both drug discovery and basic research. ION Brilliant Calcium Flex provides all the reagents necessary for use as a washed or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Ca2+ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.