Multiplex RT-PCR system detects two SARS-CoV-2 specific genes (N gene and ORF1ab) in a single one step RT-PCR reaction Primer & Probe Mixes contain all N gene, ORF1ab and human RP assays Positive Control contains the three SARS-related target genes: N gene, ORF1ab gene as well as the human RP (internal control) . Convenient ready-to-use 2X One-Step RT-PCR Master Mix Manufactured under ISO 13485:2016 for research use only CE-IVD marked version also available Find out more information about our complete COVID-19 workflow Norgen’s COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) includes 2X One-Step RT-PCR Master Mix, a primer/probe mix, a positive control and a negative control (nuclease-free water) . The primer/probe mix contains three SARS-CoV-2 detection targets; N (Nucleoprotein), ORF1ab (Open reading frame1ab) and the human RNase P transcript (RP - HEX/VIC) as an internal control target to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The provided N/ORF1ab/RP Positive Control contains an in vitro RNA transcript for the two SARS-related target genes: N gene, ORF1ab gene as well as the human RP gene (internal control) . This kit was developed and validated to be used with the following PCR instruments: Qiagen Rotor-Gene Q BioRad CFX96 Touch™ Real-Time PCR Detection System ABI 7500 ABI Quantstudio 5 and 7