For quantitative determination of acetylcholine and evaluation of drug effects on acetylcholine metabolism. Key Features: Use 20 µ L samples. Linear detection range: colorimetric assay 10 to 200 µ M, fluorimetric assay 0.4 to 10 µ M acetylcholine. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, milk, tissue, and cell culture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: OD, FL: 10, 0.4 µ M.