For quantitative determination of glutamate (monosodium glutamate or MSG) and evaluation of drug effects on glutamate metabolism. Key Features: Sensitive and accurate. Detection limit of 50 µ M, linearity up to 2.5 mM glutamate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 30 min at room temperature. No 37° C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, urine, cell, tissue etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 50 µ M.