For quantitative determination of glutamine and evaluation of drug effects on glutamine metabolism. Key Features: Sensitive and accurate. Use 20 µ L sample. Linear detection range 0.023 - 2 mM glutamine in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, incubation for 40 min at room temperature, adding a stop reagent and reading the optical density. No 37° C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, urine, cell, tissue etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 23 µ M.