For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 µ M and linearity up to 10 µ M NAD+/NADH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 15 min at room temperature. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Cell or tissue extracts. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.05 µ M.