For quantitative fluorescent immunoenzymatic assay of ERK1/2 phosphorylation status in cultured cells. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs) . Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Cell-based ELISA (FL360/450nm, 530/585nm) . Samples: Cells. Species: Human, mouse, rat. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.