For evaluation of drugs and screening of potential inhibitors to MMP-1. Robust. Fluorescence-based assay with a longer wavelength probe (λex/em = 490/520 nm), minimizing test compound interference. Robust assay with a Z’ factor of > 0.8. Homogeneous and convenient. Homogenous “mix-incubate-measure” assay. No wash and reagent transfer steps are involved. High-throughput. Can be readily automated to assay thousands of samples per day. Method: FL490/520 nm; Samples: Compounds that affect MMP-1; Species: Human; Procedures: 75 min