For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. Key Features: Sensitive and accurate. Detection limit of 0.02 µ M and linearity up to 1 µ M NAD+/NADH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: F530/585nm. Samples: Cell, tissue extracts etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.02 µ M.