For quantitative determination of tryptophan and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 10 µ L sample. Linear detection range from 10µ M to 400 µ M tryptophan in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 30 minutes. Room temperature assay. No 37° C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585nm. Samples: Serum. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 10 µ M.