<font size="3">EvaGreen&amp;reg; Dye, 2000X in DMSO is offered for customers with specialized applications requiring a concentrated dye solution.&amp;nbsp; &amp;nbsp; Note: for most qPCR applications, we recommend our ready-to-use Forget-Me-Not&amp;trade; EvaGreen&amp;reg; qPCR Master Mixes or EvaGreen&amp;reg; Dye, 20X in water.&amp;nbsp; &amp;nbsp; EvaGreen&amp;reg; dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR&amp;reg; Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen&amp;reg; dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen&amp;reg; dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR&amp;reg; Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer.&amp;nbsp; &amp;nbsp; The unique properties of EvaGreen&amp;reg; dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR&amp;reg; Green I, EvaGreen&amp;reg; dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen&amp;reg; dye can be used at a much higher dye concentration than SYBR&amp;reg; Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.&amp;nbsp; &amp;nbsp; EvaGreen&amp;reg; dye and applications are covered under granted and pending US and international patent</font>s.<br><div><br></div><div><font size="5" color="#133094"><b>Key Features </b></font> <ul><li><font size="3"><b><font color="#0015ab">2000X Concentrated Formulation → </font></b>Highly concentrated EvaGreen® dye in DMSO for specialized applications requiring flexible dye preparation.</font></li><li><font size="3"><font color="#0015ab"><b>High Fluorescence Upon DNA Binding →</b></font> Essentially non-fluorescent in solution but becomes highly fluorescent when bound to double-stranded DNA.</font></li><li><font size="3"><b><font color="#0015ab">Low PCR Inhibition →</font></b> EvaGreen® is generally less inhibitory to PCR than SYBR® Green I, allowing higher dye concentrations in suitable amplification workflows.</font></li><li><font size="3"><b><font color="#0015ab">High-Sensitivity DNA Detection → </font></b>Supports strong fluorescence signals for real-time PCR, melt curve analysis and digital PCR applications.</font></li><li><font size="3"><font color="#0015ab"><b>FAM / SYBR® Green I-Compatible Spectrum → </b></font>DNA-bound EvaGreen® has excitation and emission spectra close to fluorescein (FAM) and SYBR® Green I, supporting compatibility with instruments using appropriate detection channels.</font></li><li><font size="3"><b><font color="#0015ab">Excellent Stability →</font></b> EvaGreen® exhibits high thermal and hydrolytic stability, supporting routine laboratory handling.</font></li><li><font size="3"><b><font color="#0015ab">Suitable for Specialized Applications →</font></b> Designed for workflows including qPCR, DNA melt curve analysis, tHDA and capillary gel electrophoresis.</font></li><li><font size="3"><b><font color="#0015ab">Nonmutagenic and Noncytotoxic →</font></b> EvaGreen® has been reported as nonmutagenic and noncytotoxic based on manufacturer-reported testing.</font></li></ul></div>