<font size="3">EvaGreen® Dye, 20X in Water is a green fluorescent nucleic acid dye designed for real-time PCR (qPCR), DNA melt curve analysis, HRM®, LAMP and digital PCR. The dye is essentially non-fluorescent in its free form and becomes highly fluorescent upon binding to double-stranded DNA (dsDNA), enabling sensitive fluorescence-based DNA detection. The 20X aqueous formulation provides a convenient DNA-binding dye for qPCR workflows and is spectrally similar to SYBR® Green I/FAM, making it compatible with commonly used real-time PCR detection channels.</font><div><br><div><font size="5" style="font-weight: bold; color: rgb(19, 48, 148);">Key Features </font><ul style=""><li style=""><font size="3" style=""><font style="" color="#0015ab"><b>Low PCR Inhibition →</b></font><font color="#133094" style="font-weight: bold;"> </font><font color="#000000" style="">EvaGreen® is less inhibitory to PCR than SYBR® Green I, allowing higher dye concentrations and stronger fluorescence signals in suitable qPCR workflows.</font><font color="#133094" style="font-weight: bold;"> </font></font></li><li style=""><font size="3" style=""><font color="#0015ab"><b>High Sensitivity →</b></font><b style="color: rgb(19, 48, 148);"> </b><font color="#000000">Its low PCR inhibition enables high dye concentrations for sensitive qPCR detection and robust DNA melt curve analysis.</font><b style="color: rgb(19, 48, 148);"> </b></font></li><li style=""><font size="3" style=""><font color="#0015ab"><b>Nonmutagenic and Noncytotoxic →</b></font><b style="color: rgb(19, 48, 148);"> </b><font color="#000000">EvaGreen® has been shown to be non-mutagenic and non-cytotoxic in standard Ames testing and is impermeable to cell membranes.</font><b style="color: rgb(19, 48, 148);"> </b></font></li><li style=""><font size="3" style=""><b style="color: rgb(19, 48, 148); font-weight: bold;"><font color="#0015ab">Compatible with Fast PCR →</font></b><font color="#000000"> Minimal PCR interference supports fast PCR protocols where reduced extension times are required.</font><b style="color: rgb(19, 48, 148);"> </b></font></li><li style=""><font size="3" style=""><b style="color: rgb(19, 48, 148); font-weight: bold;"><font color="#0015ab">Multiplex PCR Compatible →</font></b><b style="color: rgb(19, 48, 148);"> </b><font color="#000000">EvaGreen® exhibits limited dye redistribution between amplicons at recommended concentrations, supporting multiplex PCR applications and melt-curve-based analysis.</font><b style="color: rgb(19, 48, 148);"> </b></font></li><li style=""><font size="3" style=""><font style=""><font color="#0015ab"><b>Exceptional Stability →</b></font><span style="font-weight: normal;"><font color="#000000"> </font></span></font><font color="#000000">The dye provides high thermal, chemical and photostability, including stability under PCR conditions.</font><b style="color: rgb(19, 48, 148);"> </b></font></li><li style=""><font size="3" style=""><b style="font-weight: bold; color: rgb(19, 48, 148);"><font color="#0015ab">SYBR® Green I-Like Spectral Profile →</font></b><font color="#133094" style="font-weight: bold;"> </font><font color="#000000" style="">EvaGreen® can be detected using common SYBR® Green I or FAM channels, facilitating compatibility with major real-time PCR platforms.</font></font></li></ul></div></div>