<font size="3">FNC Coating Mix® is a specially formulated serum-free tissue culture reagent containing fibronectin collagen and albumin that is used to enhance the attachment of adherent cells to plastic flasks or microplates. The unique formula creates an extracellular matrix that dramatically increases the rate of cell attachment to any plastic substratum. The matrix accelerates monolayer formation especially when fastidious cell types such as human prostrate and breast epithelial cells are being propagated. Mammalian cell cultures will attach and grow more effectively on FNC-coated plastic surfaces when cultured in the appropriate serum-free medium. AthenaES® offers several different serum-free media that when used in conjunction with FNC Coating Mix® increase the growth of cell cultures dramatically.</font><div><font size="3"><br></font></div><div><font size="5" color="#133094"><b style="">Key Features </b></font><font size="3"> </font><ul style="font-size: medium;"><li><font size="3"><font color="#0015ab"><b>Enhanced Cell Attachment →</b></font> Improves attachment of adherent mammalian cells to plastic culture surfaces. </font></li><li><span style="font-size: medium;"><b><font color="#0015ab">Serum-Free Coating Reagent →</font></b> Designed for cell culture workflows using appropriate serum-free culture media.</span></li><li><span style="font-size: medium;"><b><font color="#0015ab">Extracellular Matrix Components →</font></b> Contains fibronectin, collagen and albumin to provide a cell-compatible coated surface.</span></li><li><span style="font-size: medium;"><b><font color="#0015ab">Ready-to-Use Formulation →</font></b> Supplied as a liquid coating reagent for convenient preparation of culture surfaces.</span></li><li><span style="font-size: medium;"><b><font color="#0015ab">Supports Monolayer Formation → </font></b>Promotes efficient cell attachment and monolayer development in suitable adherent cell culture systems.</span></li><li><span style="font-size: medium;"><b><font color="#0015ab">Suitable for Fastidious Cell Types →</font></b> Particularly useful for adherent cells that require enhanced substrate attachment, including certain epithelial cell cultures.</span></li></ul><div style="font-size: medium;"><br></div><div style=""><font size="5" color="#133094"><b style="">Typical Coating Workflow </b></font><font size="3"> For compatible cell culture applications : <b>1. Coat →</b> Cover the culture surface completely with FNC Coating Mix®. <b>2. Incubate →</b> Allow the coating to contact the surface according to the applicable protocol. <b>3. Remove Excess →</b> Aspirate the remaining coating solution. <b>4. Seed Cells → </b>Add the prepared cell suspension to the coated culture surface. <br></font></div><div style=""><font size="3">The manufacturer's associated product information describes a coating volume of approximately 0.2 mL/cm², with the coating typically incubated for about 30 seconds before removal.</font></div></div>