Athena’s Freezing Media PairTM are a duaL- cryopreservation system used to safely store suspensions of viable cells detached from monolayer cultures using the “cold trypsin” method with Athena’s cell dissociation media PETTM. The pair of solutions thoroughly preserve cells which provides superior cell viability upon reconstitution. This Freezing Media PairTM has been used extensively to cryopreserve a variety of human cell lines grown in serum-free media including breast and prostate cancer cell lines.