Galactosylceramidase (or galactocerebrosidase), EC 3.2.1.46, is an enzyme that removes galactose from ceramide derivatives (galactosylceramides) by catalysing the hydrolysis of galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is a lysosomal protein, encoded in humans by the GALC gene. Mutations in this gene have been associated with Krabbe disease, also known as galactosylceramide lipidosis.