BRFF-HPC-1™ is a complete serum-free medium comprised of essential amino acids vitamins inorganic salts trace elements growth factors and dihydrotestosterone designed for establishing new cell lines from human prostate tissue. HPC-1™ was developed and has been extensively used for establishing and maintaining epithelial cell lines from both benign prostatic hyperplasia and prostatic carcinoma. Optimal attached monolayer cell growth can be achieved when used in conjunction with FNC Coating Mix®.