No wash, ratiometric calcium flux assay, 2 plates. Total assay solution compatible with plate reader and fluorescence microscopy applications. Excitation: 340, 380 nm | Emission: 505 nm | Kd: 145 nM Ratiometric Calcium Essentials kit provides the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give the user the flexibility needed to customize your assay.