The PDQ™ Protease Assay is a unique colorimetric assay used to detect protease activity in aqueous samples. The proprietary substrate responds to a wide range of proteases including serine metallo aspartate and cysteine proteases such as collagenase proteinase K papain pepsin bromelin ficin trypsin and chymotrypsin. PDQ™ can be used with just a few simple steps to measure protease activity and requires no centrifugation. The substrate is a cross-linked matrix containing protein substrate and a dye-protein conjugate. Protease activity is detected spectrophotometrically with increasing optical density proportional to increasing enzyme activity and can detect nanogram quantities. Each kit is supplied with a trypsin solution for generating standard curves (BAEE equivalent units) . PDQ™ is supplied in 48 ready to use plasict vials.