​PET™ is a superior cell dissociation reagent specially formulated for use under “Cold Trypsin” conditions. The unique blend of polyvinylpyrrolidone EGTA and trypsin in a HBS base allows the trypsin to retain enzymatic activity under cool temperatures unlike standard trypsin which requires a temperature of 37°C. When used to detach monolayer cultures of epithelial cells from the growth substratum this reagent yields more viable cells from monolayers grown in serum-free medium than traditional trypsin formulas.