E. coliKit contents: PMA dye, 20 mM in water, 100 uL 5X PMA Enhancer for Gram Negative Bacteria, 16 mL 2X Forget-Me-NotTM qPCR Master Mix, 2 x 1 mL (200 reactions) ROX reference dye, 1 mL Z3276 primer mix, 5 uM, 400 uL PMA dye, 20 mM in water, 100 uL 5X PMA Enhancer for Gram Negative Bacteria, 16 mL 2X Forget-Me-NotTM qPCR Master Mix, 2 x 1 mL (200 reactions) ROX reference dye, 1 mL Z3276 primer mix, 5 uM, 400 uL E. coliEscherichia coliE. coliE. coliE. coliuidAM/em> kit. We also offer E. coli viability PCR kits with PMAxxTM dye, a new and improved version of PMA designed by Biotium scientists to be a superior alternative to PMA. While PMA is generally effective at differentiating between live and dead bacteria by qPCR, it does not completely eliminate PCR products from dead cell DNA. This could potentially give false positive results. Biotium's new dye PMAxxTM is much more effective at eliminating PCR amplification of dead cell DNA, and therefore provides the best discrimination between live and dead bacteria. Materials from Biotium are sold for research use only.E. coli