For quantitative determination of biotin in food, cosmetic, and biotinylated proteins. Key Features: Fast and sensitive. Linear detection range: 8 to 200 µ M biotin with 30 µ L sample (96-well) or 10 to 200 µ M biotin with 10 µ L sample (384-well) . Convenient. The procedure involves adding a single working reagent and reading after 20 minutes. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD500nm. Samples: Food, cosmetics, supplements, and biotinylated proteins or antibodies. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 8 µ M.