For quantitative determination of reduced glutathione (GSH) and evaluation of drug effects on glutathione metabolism. Key Features: Sensitive and accurate. Linear detection range 0.4 - 100 µ M in 96-well plate. Simple and convenient. The procedure involves mixing the DTNB Reagent with sample, removing protein precipitates for proteinaceous samples, adding a second Reagent and reading the optical density. Low interference. Amino acids and common buffers do not interfere. Method: OD412nm. Samples: Whole blood, plasma, serum, urine, tissue and cell extracts. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 12 µ g/dL (0.4 µ M) .