For quantitative determination of hydroxyproline and collagen in biologic and cosmetic samples. Key Features: Safe. Our hydroxyproline assay uses an improved perchlorate-free chemistry. Sensitive and accurate. Uses 20 µ L sample. Linear detection range 0.5 µ g/mL to 50 µ g/mL hydroxyproline in 96-well plate assay. Convenient. Collagen can be hydrolyzed in Eppendorf tubes, and final incubation can be performed at 37° C. Method: OD560nm. Samples: Hydroxyproline and collagen in biologic and cosmetic samples. Species: All. Procedure: Assay takes Approx. 2 hrs. Kit size: 100 Tests. Detection limit: 0.5 µ g/mL.