For high-throughput inhibitor screening and evaluation of pyrophosphatase modulators. Key Features: Safe. Non-radioactive assay. Fast and convenient. The procedure involves incubation of enzyme and inhibitor, addition of a single working reagent and incubation for 30 min. Room temperature assay. No 37°C incubator is needed. High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems. Robust assay with a Z’ factor of 0.59. Can be used in 96-well, 384-well, and potentially higher density screening assays. Method: OD620; Samples: Test compounds; Species: All; Procedures: 70 min; Size: 100 tests in 96well plate